ENZYMES: PENICILLIN G ACYLASE
Both 6-Aminopenicillanic acid (6-APA) and 7-Aminodeacetoxycephalosporanic
acid (7-ADCA) are key intermediates used for the production of semi-synthetic
Penicillins and Cephalosporins.
Some years ago 6-APA and 7-ADCA were predominantly produced by chemical
means. This method was associated with a number of complex technical
requirements, and used materials that caused environmental pollution
as well as producing unwanted impurities in the product.
The Enzyme route, which is used as an alternative, provides many
advantages over the chemical route that includes:
Reduced reaction time - increased operating efficiency
Specificity - the production of unwanted by-products
is avoided and there is no need to extensively refine and purify
the desired product
Cost saving - enzymes can be immobilized and therefore
re-used several times, providing valuable cost savings
Environmentally friendly - enzymes are non corrosive
and fully biodegradable
The official nomenclature for Penicillin Acylase/Amidase
(Enzyme) is E.C.220.127.116.11, and is derived from Escherichia coli.
Dalas Biotech has developed a method to produce Penicillin
G Acylase on an industrial scale, that is free of -lactamase
and other contaminants.
The PENAM and CEPHAM class of -Lactam
antibiotics, of Penicillins and Cephalosporins, are the most widely
used antibiotics today.
The key intermediates 6-APA and 7-ADCA are obtained by the enzymatic
deacylation of Penicillin G Potassium (Pen GK) and Cephalosporin
G (Ceph G) respectively.
The enzymatic conversion is brought about by the cleavage of a side
chain of the molecule, in a highly specific manner:
We have developed several versions of the Penicillin G Acylase enzyme
in-house, as a result of the efforts of our research and development
team. The two main versions do have their specific independent advantages,
but share some similarities as well:
1. Black Enzyme
2. "Dalenz" White Enzyme
The Black Enzyme:
i. A well-established biocatalyst, successfully applied by many
of our customers.
ii. Its key benefits lie in its significantly higher activity, and
resistance to solvents.
Our latest development Dalenz:
i. The advantage of Dalenz over other biocatalysts lies in its stability
- an attribute of its covalent bonding to a carrier.
ii. The Dalenz white enzyme combines both high specific activity,
with unmatched stability of the catalyst under process conditions.
Although enzymes provide a great number of advantages over chemicals,
it is important to maintain certain conditions for the most efficient
output. For the Dalas Penicillin G Acylase enzymes, we suggest the
Loading Per Kg. of Enzyme
Expected Reaction Time
D.M. Water Quality
should be less Than 20 ÁS, and free from both Chlorides and